ABSTRACT
OBJECTIVE: To establish the HPLC fingerprints of Fructus Aristolochiae and honey-fried products from different habitats, compare and analyze their difference in chemical components. METHODS: Using an Agilent ZORBAX SB-C18 column (4.6 mm×250 mm, 5 μm), HPLC method was performed to collect the fingerprints. The mobile phase A and B were acetonitrile and 1% acetic acid, respectively. Gradient elution was performed at the flow rate of 1.0 mL·min-1 and column temperature of 30℃. The detection was carried out at 250 nm. Similarity Evaluation System for Chromatographic Fingerprint of TCMs (Ver. 2012) and SPSS22.0 was used in cluster analysis and principal component analysis (PCA). RESULTS: The fingerprints of 14 batches of Fructus Aristolochiae samples, before and after process, were established by HPLC. The reduction rate of AA I, a poisonous component, was over 36%. Except for S4 (0.822), S18 (0.771) and S19 (0.639), the similarities of Fructus Aristolochiae and processed products were over 0.9. The non-processing samples were classified into four groups by cluster analysis. As PCA shown, the five principal components including AA I had a contributing rate of 84.914% to cumulative sum of squares, illustrating the chemical similarity and difference of samples from different areas. CONCLUSION: The results may provide reference for Fructus Aristolochiae processing and standardization of its detoxification technology.
ABSTRACT
Objective To optimize static pressurized liquid extraction(PLE) method for the extraction of aristolochic acids Ⅰ and Ⅱ(AAⅠ and AAⅡ) from Fructus Aristolochiae and study the influences of related factors.Methods The univariate design was introduced.The operational parameters,such as the type of solvent,particle size of the sample powder,extraction temperature,pressure,static time,flush volume,the number of cycles,and the amount of sample were optimized.Results The optimized result employed methanol as extraction solvent,particle size of 100—120 meshes,extraction temperature of 120 ℃,extraction pressure of 10.3 MPa,static time of 10 min,flush volume of 40%,1 cycle,and sample amount of 1.00 g.The method was applied for four species of traditional Chinese medicinal materials including Fructus Aristolochiae,Caulis Aristolochiae Manshuriensis,Radix Aristolochiae,and Radix Arsitolochiae Fangchi.Conclusion This method can be used to completely extract AAⅠ and AAⅡ from Fructus Aristolochiae in once extraction.The comparison shows that this static PLE method is better than ultrasonication and Soxhlet methods with higher extraction efficiency and less time-consuming.It is also better than the dynamic one in the extraction of AAs from Radix Aristolochiae.